1) Before washing one’s hands one should not touch any opening in one’s body. Examples of these are the mouth, nose, ears etc. The reason for this is that the evil spirit which rest son the hands can damage these limbs. One should, if possible, endeavor not to touch one’s eyes even from outside. (Shulchan Aruch and Mishne Berura 4)
2) One should be very careful not to touch any food before washing one’s hands, and in the event that he did touch, the food item should be washed three times. (Mishne Berura ibid)